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About this Article
Written by: Monica Jain
Written on: December 6th, 2011
Tags: biomedical engineering, communication, electrical engineering, computer science, health & medicine, physics
Thumbnail by: National Institutes of Health/Wikimedia Commons
About the Author
Monica Jain is a junior studying biomedical engineering at the Viterbi School of Engineering at University of Southern California. She loves to cook, socialize, and go on adventures and hopes to one day own her own restaurant.
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Volume XIV Issue I > Mobile Microscopes: How Your Cell Phone Can Save Lives
What if a text message could save a life? Dr. Ayogdan Ozcan and his team of researchers have developed a cost-efficient, revolutionary device that can perform basic diagnostics for blood cell count, malaria, and tuberculosis – all on the back of a $30 camera phone. The device uses a lens-free imaging technique known as LUCAS, which creates a holographic image of each sample cell based on their imaged “shadows”. The shadows are imaged by diffracting a light beam through the cells and upon a CCD chip, which collects the data for advanced image processing. These holograms can then be compared to a holographic cell database using a simple pattern-matching algorithm that counts the cells and diagnoses any potential diseases. The diagnostic results are sent back via text message to the original patient or doctor in a matter of minutes. This device is revolutionizing the fields of microscopy and mobile health technology and may one day turn the cell phone into an essential local and global medical device.

Introduction

Imagine if you could perform a complete blood cell count and diagnose deadly bacterial diseases – on the back of your cell phone. Well, imagine no more.
An inexpensive, lightweight device is now being developed that will allow doctors and health workers to do just that: perform basic medical diagnostics on the back of a $30 cell phone. This camera phone attachment is a small digital microscope that, costing under $10 to produce, can perform many medical tests that normally require expensive lab equipment and trained personnel who can conduct tests such as blood cell count, identification of diseased cells, water contamination detection, and diagnosis of infectious diseases, including malaria, cholera, and tuberculosis (TB).
It is a lightweight, cost-effective, and lens-free microscope that uses computer algorithms instead of expensive and bulky lenses to extract an extensive set of biological information from simple, camera phone images.

The Global Cell Phone Network

Twenty years ago, a cell phone could do little else besides make a phone call. Today, however, the cell phone has become the Swiss Army knife of modern technology. Few phones are without Internet access, accelerometers, global positioning systems (GPS), or video cameras. It comes as no surprise, then, that the cell phone is now being retooled into a medical device as well.
russavia/Wikimedia Commons
Figure 1: Resource-poor countries such as Uganda and India do not have ready access to phones that might save their lives.
What is surprising, however, is the ubiquity of cell phones. Today, nearly 5 billion people around the world own a cell phone [1], a majority of whom live in resource-poor countries such as Uganda and India (Fig. 1). Almost 90% of the world’s population is covered by a mobile network, and by 2015, it is estimated that 90% of the world’s population will carry a network-subscribed cell phone [2]. As Professor Ramesh Raskar of the Massachusetts Institute of Technology Media Labs notes, “People who make a dollar a day have a cell phone, which is just mind-blowing if you think about it [3].” These statistics alone make it one of the most accessible resources for innovation and healthcare intervention.
Dr. Aydogan Ozcan and his team of researchers at the Electrical Engineering lab in the University of California, Los Angeles (UCLA) have figured out a way to tap into this substantial resource and provide healthcare to the masses with a revolutionary, lens-free cell phone attachment that can bring effective diagnostics to parts of the world that cannot access expensive clinical equipment and laboratories.

The Inner Workings of A Revolutionary Mobile Device

LEDs, CCDs, and Diffraction Signatures

The device is composed of two key hardware components: a light-emitting diode (LED) that illuminates the sample and a charged-coupled device (CCD) chip, identical to that found in a consumer digital camera. Slides containing a blood or water sample are loaded into the phone in between the LED and the light-sensing chip, “just like you insert a memory stick [4],” says Ozcan. Each slide is then illuminated by the LED from above; as the light passes through the given cell type, it diffracts or bends in a characteristic way. “What we record is not an image but a diffraction signature,” says Ozcan [5]. Each cell type has a unique diffraction signature based upon its size, shape, and refractive index [5].
Bill Hammack/Wikimedia Commons
Figure 2: The n- and p-type substrates provide and store electrons, respectively.
The CCD chip then collects the diffraction signatures through capacitance, the ability of electrical devices such as the chip to store energy—in this case, the diffracted light waves. Capacitance in the CCD chip operates via the gathering of electric charge, or electrons. The chip is made up of an array of light-sensitive pixels, or CCD gates, which are composed of perpendicular strips of transparent electrode material—silicon dioxide—and n-type silicon channels (n-Channels) embedded within a p-type silicon substrate [6] (Fig. 2). N-type silicon is a semiconductor capable of providing extra electrons, which the p-type silicon, normally electron-deficient, can accept and store. Points at which the n-channels cross the electrode material are known as CCD gates and correspond to one pixel.
When a photon of light strikes a particular gate, it dislodges electrons from the n-channels, which causes the formation of potential barriers and wells within the p-type substrate. The excited electrons remained trapped in these wells, and each gate begins to accumulate an electric charge in proportion to the intensity of light at that gate. Once the image exposure is complete, the CCDs operate as a shift register and transfer collected charges to the end of the column for conversion and digitized imaging [7].

LUCAS and Digital Holography

The CCD chip is part of the underlying technology behind the device known as LUCAS: Lens-free Ultra-wide field of view Cell monitoring Array platform based on Shadow imaging. Instead of directly imaging and magnifying cells with a lens, as a conventional microscope does, LUCAS uses the interferometric, or electromagnetic wave-based, technique to image cell shadows in a process known as digital holographic microscopy [8]. Unlike the dark shadows of the macro-world, such as our own, the shadows - or diffraction signatures - of micro-objects, like that of a cell or bacterium, contain an extremely rich source of quantified information that accurately describes the object’s spatial features [9]. These micro-scale shadows are not flat, but rather, rippled in texture, the details of which constitute a holographic image of the cell [10]. This hologram acts as the cell’s fingerprint and can be used to detect the cell type and potential deformations such as sickle-cell anemia and malarial disease.